Professor Nick Hastie: Medical and Developmental Genetics

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Cancer, Development and Adult Tissue Maintenance


 

  1. Menke, A.L.; Clarke, A.R.; Leitch, A.; Ijpenberg, A.; Williamson, K.A.; Spraggon, L.; Harrison, D.J. and Hastie, N.D. Genetic interactions between the Wilms' tumor 1 gene and the p53 gene.
    Cancer Res 62(22):6615-6620, 2002 PubMed Abstract
  2. Hastie, N.D. Life,Sex, and WT1 Isoforms- Three Amino Acids Can Make All the Difference. Cell 106:391-394, 2001
    PubMed Abstract
  3. Little, N.; Hastie, N.D. and Davies, R.C. Identification of WTAP, a novel Wilms' tumour 1-associating protein. Human Molecular Genetics 9(15):2231-2239, 2000 PubMed Abstract
  4. Davies, R.C.; Bratt, E. and Hastie, N.D. Did nucleotides or amino acids drive evolutionary conservation of the WT1 +/-KTS alternative splice? Human Molecular Genetics 9(8):1177-1183, 2000 
    PubMed Abstract
  5. Ladomery, M.R.; Slight, J.; McGhee, S. and Hastie, N.D. Presence of WT1, the Wilm's Tumour Suppressor Gene Product, in Nuclear Poly(A)+ Ribonucleoprotein.
    J Biol Chem 274(51):36520-36526, 1999
    PubMed Abstract
  6. Patek, C.E.; Little, M.H.; Fleming, S.; Miles, C.; Charlieu, J.P.; Clarke, A.R.; Miyagawa, K.; Christie, S.; Doig, J.; Harrison, D.J.; Porteous, D.J.; Brookes, A.J.; Hooper, M.L. and Hastie, N.D. A zinc finger truncation of murine WT1 results in the characteristic urogenital abnormalities of Denys-Drash syndrome.
    Proc Natl Acad Sci 96(6):2931-2936, 1999 
    PubMed Abstract
  7. Moore, A.W.; McInnes, L.; Kreidberg, J.; Hastie, N.D. and Schedl, A. YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis.
    Development 126(9):1845-1857, 1999 PubMed Abstract
  8. Moore, A.W.; Schedl, A.; McInnes, L.; Doyle, M.; Hecksher-Sorensen, J. and Hastie, N.D. YAC transgenic analysis reveals Wilms' tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb.
    Mech Dev79(1-2):169-184, 1998
    PubMed Abstract
  9. Miyagawa, K.; Kent, J.; Moore, A.; Charlieu, J.P.; Little, M.H.; Williamson, K.A.; Kelsey, A.; Brown, K.W.; Hassam, S.; Briner, J.; Hayashi, Y.; Hirai, H.; Yazaki, Y.; van Heyningen, V. and Hastie, N.D. Loss of WT1 function leads to ectopic myogenesis in Wilms' tumour [letter].
    Nature Genetics 18(1):15-17, 1998
    PubMed Abstract
  10. Davies, R.C.; Calvio, C.; Bratt, E.; Larsson, S.H.; Lamond, A.I. and Hastie, N.D. WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes.
    Genes Dev 12(20):3217-3225, 1998 PubMed Abstract
  11. Larsson, S.H.; Charlieu, J.P.; Miyagawa, K.; Engelkamp, D.; Ross, A.; van Heyningen, V.; Hastie, N.D.; Cuzin, F. and Rassoulzadegan, M. Subnuclear Localization of WT1 in Splicing or Transcription Factor Domains is Regulated by Alternative Splicing. Cell 81:391-401, 1995
    PubMed Abstract
  12. Hastie, N. D. The genetics of Wilms' tumor - a case of disrupted development.
    Annual Review Genet 28:523-558, 1994 PubMed Abstract
  13. Bickmore, W.A.; Oghene, K.; Little, M.H.; Seawright, A.; van Heyningen, V. and Hastie, N.D. Modulation of DNA binding specificity by alternative splicing of the Wilms tumor wt1 gene transcript.
    Science 257:235-237, 1992
    PubMed Abstract
  14. Armstrong, J.F.; Pritchard-Jones, K.; Bickmore, W.A.; Hastie, N.D. and Bard, J.B.L. The expression of the Wims' tumour gene, WT1, in the developing mammalian embryo.
    Mechanisms Dev 40:85-97, 1992
    PubMed Abstract
  15. van Heyningen, V.; Bickmore, W.A.; Seawright, A.; Fletcher, J.M.; Maule, J.; Fekette, G.; Gessler, M.; Bruns, G.A.P.; Huerre-Jeanpierre, C.; Junien, C.; Williams, B.R.G., and Hastie, N.D. Role for the Wilms tumor gene in genital development.
    Proc Natl Acad Sci 87:5383-5386, 1990
    PubMed Abstract
  16. Pritchard-Jones, K.; Fleming, S.; Davidson, D.; Bickmore, W.; Porteous, D.; Gosden, C.; Bard, J.; Buckler, A.; Pelletier, J.; Housman, D.; van Heyningen, V. and Hastie, N. The candidate Wilms' tumour gene is involved in genitourinary development. Nature 346:194-197, 1990 PubMed Abstract

 

  1. Molecular functions of WT1
  2. WT1 in kidney development
  3. Wt1 and Wnt4
  4. Wt1 outside the Kidney
  5. WT1 in MET and EMT
  6. Wt1 in Cancer
  7. Publications (this page)

 

Key Publications

  1. Martinez-Estrada, O.M.; Lettice, L.A.; Essafi, A.; Guadix, J.A.; Slight, J.; Velecela, V.; Hall, E.; Reichmann, J.; Devenney, P.S.; Hohenstein, P.; Hosen, N.; Hill, R.E.; Munoz-Chapuli, R. and Hastie, N.D. Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of Snail and E-cadherin Nat.Genet. 42(1):89-93, 2010
    PubMed Abstract
  2. Dudnakova, T.; Spraggon, L.; Slight, J. and Hastie, N. Actin: a novel interaction partner of WT1 influencing its cell dynamic properties. Oncogene e-pub Dec 7, 2009 PubMed Abstract
  3. Hohenstein, P.; Slight, J.; Ozdemir, D.D.; Burn, S.F.; Berry, R., and Hastie, N.D. High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi.
    Pathogenetics 1(1):3, 2008
    PubMed Abstract
  4. Vitart, V.; Rudan, I.; Hayward, C.; Gray, N.K.; Floyd, J.; Palmer, C.N.; Knott, S.A.; Kolcic, I.; Polasek, O.; Graessler, J.; Wilson, J.F.; Marinaki, A.; Riches, P.L.; Shu, X.; Janicijevic, B.; Smolej-Narancic, N.; Gorgoni, B.; Morgan, J.; Campbell, S.; Biloglav, Z.; Barac-Lauc, L.; Pericic, M.; Klaric, I.M.; Zgaga, L.; Skaric-Juric, T.; Wild, S.H.; Richardson, W.A.; Hohenstein, P.; Kimber, C.H.; Tenesa, A.; Donnelly, L.A.; Fairbanks, L.D.; Aringer, M.; McKeigue, P.M.; Ralston, S.H.; Morris, A.D.; Rudan, P.; Hastie, N.D.; Campbell, H. and Wright, A.F. SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout. Nat Genet 40(4):437-442, 2008 PubMed Abstract
  5. Ijpenberg, A.; Perez-Pomares, J.M.; Guadix, J.A.; Carmona, R.; Portillo-Sanchez, V.; Macias, D.; Hohenstein, P.; Miles, C.M.; Hastie, N.D. and Munoz-Chapuli, R. Wt1 and retinoic acid signaling are essential for stellate cell development and liver morphogenesis.
    Dev Biol     312(1):157-170, 2007
    PubMed Abstract
  6. Spraggon, L.; Dudnakova, T.; Slight, J.; Lustig-Yariv, O.; Cotterell, J.; Hastie, N. and Miles, C. hnRNP-U directly interacts with WT1 and modulates WT1 transcriptional activation.
    Oncogene     26(10):1484-1491, 2007
    PubMed Abstract
  7. Hohenstein, P. and Hastie, N.D. The many facets of the Wilms' tumour gene, WT1. Hum Mol Genet 15 Suppl 2:R196-R201, 2006
    PubMed Abstract
  8. Wilm, B.; Ipenberg, A.; Hastie, N.D.; Burch, J.B. and Bader, D.M. The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature. Development 132(23):5317-5328, 2005 PubMed Abstract
  9. Niksic, M.; Slight, J.; Sanford, J.R.; Caceres, J.F. and Hastie, N.D. The Wilms' tumour protein (WT1) shuttles between nucleus and cytoplasm and  is present in functional polysomes.
    Hum Mol Genet 13(4):463-471, 2004 PubMed Abstract
  10. Davies, J.A.; Ladomery, M.; Hohenstein, P.; Michael, L.; Shafe, A.; Spraggon, L. and Hastie, N. Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation.
    Hum Mol Genet 13(2):235-246, 2004 PubMed Abstract
  11. Patek, C.E.; Fleming, S.; Miles, C.G.; Bellamy, C.O.; Ladomery, M.; Spraggon, L.; Mullins, J.; Hastie, N.D. and Hooper, M.L. Murine Denys-Drash syndrome: evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis.
    Hum Mol Genet 12(18):2379-2394, 2003 PubMed Abstract
  12. Miles, C.; Rankin, L.; Smith, S.I.; Niksic, M.; Elgar, G. and Hastie, N.D. Faithful expression of tagged Fugu WT1 protein from a genomic transgene in zebrafish: efficient splicing of pufferfish genes in zebrafish but not mice. Nucleic Acids Research 31(11):2795-2802, 2003
    PubMed Abstract
  13. Miles, C.; Slight, J.; Spraggon, L.; O'Sullivan, M.; Patek, C. and Hastie, N.D. Mice lacking the 68 amino acid, mammalian-specific, N-terminal extension of WT1 develop normally and fertile. Molecular & Cellular Biology 23(7):2608-2613, 2003
    PubMed Abstract
  14. Ortega, A.; Niksic, M.; Bachi, A.; Wilm, M.; Sanchez, L.; Hastie, N. and Valcarcel, J. Biochemeical Function of Female-Lethal (2)D/Wilms' Tumor Suppressor-1-associated Proteins in Alternative Pre-mRNA Splicing. Journal of Biological Chemistry 278(5):3040-3047, 2003 PubMed Abstract
  15. Menke, A.L.; Ijpenberg, A.; Fleming, S.; Ross, A.; Medine, C.N.; Patek, C.E.; Spraggon, L.; Hughes, J.; Clarke, A.R. and Hastie, N.D. The wt1-heterozygous mouse; a model to study the development of glomerular sclerosis.
    J Pathol 200(5):667-674, 2003
    PubMed Abstract