DR GRZEGORZ KUDLA
Chromosomes and Gene Expression
Wellcome Trust Research Career Development Fellow
|Telephone:||+44 (0)131 650 7093|
|Fax:||+44 (0)131 467 8456|
|Address:||MRC Human Genetics Unit MRC IGMM, University of Edinburgh Western General Hospital, Crewe Road, Edinburgh EH4 2XU|
|Research Programme:||Post-transcriptional regulation of gene expression|
Sequence elements in coding regions of genes play an important, but often underestimated, role in the regulation of gene expression. We are particularly interested in synonymous mutations, which do not change protein sequence, but can influence mRNA and protein levels. Our goal is to understand the role of such mutations in eukaryotic cells. To achieve this, we construct combinatorial libraries of mutated genes, and measure gene expression and function using high-throughput assays. We also develop new methods to study the roles of protein-RNA and RNA-RNA interactions in gene regulation.
- Kudla G, Granneman S, Hahn D, Beggs JD, Tollervey D: Cross-linking, ligation, and sequencing of hybrids reveals RNA-RNA interactions in yeast. Proc Natl Acad Sci U S A 108:10010-10015, 2011.
- Plotkin JB, Kudla G: Synonymous but not the same: the causes and consequences of codon bias. Nat Rev Genet 12:32-42, 2011.
- Kudla G, Murray AW, Tollervey D, Plotkin JB: Coding-sequence determinants of gene expression in Escherichia coli. Science 324:255-258, 2009.