Research Biographies

DR GRZEGORZ KUDLA

Chromosomes and Gene Expression

Wellcome Trust Research Career Development Fellow

Contact Details

Research Areas

Sequence elements in coding regions of genes play an important, but often underestimated, role in the regulation of gene expression. We are particularly interested in synonymous mutations, which do not change protein sequence, but can influence mRNA and protein levels. Our goal is to understand the role of such mutations in eukaryotic cells. To achieve this, we construct combinatorial libraries of mutated genes, and measure gene expression and function using high-throughput assays. We also develop new methods to study the roles of protein-RNA and RNA-RNA interactions in gene regulation.

Key/Selected Publications

• Kudla G, Granneman S, Hahn D, Beggs JD, Tollervey D: Cross-linking, ligation, and sequencing of hybrids reveals RNA-RNA interactions in yeast. Proc Natl Acad Sci U S A 108:10010-10015, 2011.
  PubMed Abstract
  
• Plotkin JB, Kudla G: Synonymous but not the same: the causes and consequences of codon bias. Nat Rev Genet 12:32-42, 2011.
  PubMed Abstract
  
• Wlotzka W, Kudla G, Granneman S, Tollervey D: The nuclear RNA polymerase II surveillance system targets polymerase III transcripts. EMBO J 30:1790-1803, 2011.
  PubMed Abstract
  
• Kudla G, Murray AW, Tollervey D, Plotkin JB: Coding-sequence determinants of gene expression in Escherichia coli. Science 324:255-258, 2009.
  PubMed Abstract
  
• Granneman S, Kudla G, Petfalski E, Tollervey D: Identification of protein binding sites on U3 snoRNA and pre-rRNA by UV cross-linking and high-throughput analysis of cDNAs. Proc Natl Acad Sci U S A 106:9613-9618, 2009.
  PubMed Abstract
  
• Kudla G, Lipinski L, Caffin F, Helwak A, Zylicz M: High guanine and cytosine content increases mRNA levels in mammalian cells. PLoS Biol 4:e180, 2006.
  PubMed Abstract